I've just completed a series of cool single-cell experiments using the Fluidigm high-throughput microfluidic platform. Just before my arrival at Sutton, a colleague of mine had set up an application that allows to analyse a lot of interesting genetic changes at single-cell resolution. The method has been described in detail here. In my project, I've we used it for exploring the subclonal architectures and phylogenetic histories of leukemias developed in monozygotic twin siblings. These cancers have a common in utero origin and it was interesting to see how differently cancer can evolve on the same genetic background.
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